Reverse flow injection spectrophotometric determination of iron(III) using chlortetracycline reagent

Abstract

A simple reversed flow injection colourimetric procedure for determining iron(III) was proposed. It is based on the reaction between iron(III) with chlortetracycline, resulting in an intense yellow complex with a suitable absorption at 435 nm. A 200 μl chlortetracycline reagent solution was injected into the phosphate buffer stream (flow rate 2.0 ml min −1) which was then merged with iron(III) standard or sample in dilute nitric acid stream (flow rate 1.5 ml min −1). Optimum conditions for determining iron(III) were investigated by univariate method. Under the optimum conditions, a linear calibration graph was obtained over the range 0.5–20.0 μg ml −1. The detection limit (3 σ) and the quantification limit (10 σ) were 0.10 and 0.82 μg ml −1, respectively. The relatives standard deviation of the proposed method calculated from 12 replicate injections of 2.0 and 10.0 μg ml −1 iron(III) were 0.43 and 0.59%, respectively. The sample throughput was 60 h −1. The proposed method has been satisfactorily applied to the determination of iron(III) in natural waters.