Abstract
Voltage-driven DNA translocation through nanopores has attracted wide interest for manypotential applications in molecular biology and biotechnology. However, it is intrinsicallydifficult to control the DNA motion in standard DNA translocation processes in which astrong electric field is required in drawing DNA into the pore, but it also leads touncontrollable fast DNA translocation. Here we explore a new type of DNA translocation.We dub it ‘reverse DNA translocation’, in which the DNA is pulled through a nanoporemechanically by a magnetic bead, driven by a magnetic-field gradient. This technique iscompatible with simultaneous ionic current measurements and is suitable for multiplenanopores, paving the way for large scale applications. We report the first experiment ofreverse DNA translocation through a solid-state nanopore using magnetic tweezers.
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