Reversal of drug resistance in multidrug-resistant human lung cancer cells by MRP antisense RNA mediated by retrovirus

Abstract

DRUG resistance of tumor is a very common phenomenon in clinical tumor treatment. Most patients with lung cancers, one of the most common malignant tumors in China, have no response to chemotherapy, so it is very important to study the resistant biology of lung cancer for treatment. The rate of drug sensitivity in non-small cell lung cancer (NSCLC) is lower than in small cell lung cancer (SCLC). The drug resistance, besides the effects of MDRl genet'] and GST-X'~], is also closely correlated with the effect of another novel gene MRP (multidrug resistance-related gene)[31. In the previous study on a doxorubicin-selected human NSCLC cell line, GAOK, we found that MDRl gene overexpressed and MDRl antisense RNA mediated by retrovirus could not completely reverse the phenotype of multidrug resistance['], and MRP gene and its coding product Mrp (multidrug resistance protein) also overexpressed. In the present study, in order to understand the effect of MRP in GAOK cells and possibility of reversing the multidrug resistance, we introduced MRP antisense RNA mediated by retrovirus into GAOK cells, and observed its expression and the change of multidrug resistance.