Reversal of axonal transport: similarity of proteins transported in anterograde and retrograde directions.

Abstract

Reversal of axonal transport of endogenous labeled protein was studied in intact and injured nerve axons. Nerve crushes were used to collect labeled protein transported in anterograde and retrograde directions in rat sciatic nerve motoneuron axons after administration of L-[35S]methionine to the vicinity of the cell bodies. The collected proteins were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis and subsequent fluorography. In injured nerves, where the nerves were ligated distally at the time of precursor injection, the polypeptide composition of proteins moving in anterograde and retrograde directions, 9-11 h after precursor injection, was identical, indicating that reversal at a ligature is a nonselective process. In intact nerves, protein moving in the anterograde direction 22-24 h after injection was different from that found 9-11 h after injection, and was also different from protein moving in the retrograde direction 22-24 h after injection. However, protein moving in the retrograde direction 22-24 h after injection was similar to protein moving in the anterograde direction 9-11 h after injection. Thus it appears that the same group of proteins originally transported into the axon are later returned toward the cell body. In intact axons, also, reversal was nonselective, except that one major labeled polypeptide was reduced in amount in the protein moving in the retrograde direction.