Reversal effect study of vitamin D on leukemia multidrug resistance cells

Abstract

Objective To investigate the reversal effect of vitamin D on drug resistance leukemia cell lines Jurcat vitamin D/ADR, K562/ADR and its mechanism. Methods Non-toxic concentration of vitamin D was chosen by methyl thiazolyl tetrazolium, and on line concentration experiment; the contents of vitamin D for soft erythromycin in the cell and the cell surface of P-glycoprotein(P-gp) expression level were detected by using flow cytometry instrument and vitamin D for intracellular mdr1 mRNA and mrp1 mRNA expression was detected by Real-time polymerase chain reaction; vitamin D for the change of intra cellular glutathione glycosides peptide(GSH) content was detected by adoping biochemistry method. Results Resistance reversal in experiments showed that vitamin D could decrease soft erythromycin in the accumulation of drug resistant cells, with a dose-dependent relationship; vitamin D treatment Jurcat/ADR, K562/ADR cells after 48 h, chemotherapy drugs could increase the cell concentration, and reduce P-gp and the expression of mdr1 and mrp1 gene; vitamin D treatment Jurcat/ADR, K562/ADR after 48 h could decrease the intracellular GSH content in K562, K562/ADR, Jurcat and Jurcat/ADR. Conclusions Vitamin D has the drug resis-tance reversal agents to Jurcat/ADR, K562/ADR, which might be relevant to the inhibition of cell surface of P-gp proteins function and expression and reduction of the mdr1 and mrp1 expressions of resistance genes and reduction of intracellular GSH content.Vitamin D by influencing the above mechanism, which increases the drug concentration in cells, can effectively kill the tumor cells. Key words: Vitamin D; Leukemia multidrug resistance cells; Multidrug resistance; Reversal