Revelation of Epistasis through Triple Test Cross (TTC) Analysis in Rice (Oryza sativa L.)

Abstract

Background: An investigation was performed to identify epistasis, additive, dominance components of genetic variation and yield and yield variability attributing characteristics by triple test cross testing involving three testers (P1, P2 and F1) and ten rice lines.Methods: The study materials consisted of F1 seeds of three crosses, involving six parents namely, ASD16, ADT47, ASD18, CO51, TKM9 and MTU 7029. They are evaluated in randomized complete block design with three replications. Observations were reported for seven traits, namely plant height, number of tillers per plant, number of productive tillers per plant, length of panicle, number of grains per panicle, weight of 1000 grains and yield of grain per plant on five randomly selected plants per replication.Result: The segregating population of three crosses exhibited wide range of variability for most of the traits. The difference between GCV and PCV was low for most of the characters indicated less influence of environment. Among the three crosses ASD18 x CO15 recorded high percent of heritability and genetic advance for grain yield per plant. The estimate of total epistasis revealed that i type of epistasis (additive x additive) was highly significant for number of tillers per plant, number of productive tillers per plant, panicle length and 1000 grain weight. The effect of the additive (D) variance was very important for all the traits except the number of grains per panicle. Across all traits, the degree of dominance (H / D)1/ 2 was less than unity ( less than 1) suggesting, partial of dominance. Since, the pre dominance component of epistasis in autogamous crop is additive x additive (i type), it was suggested that the selection may be post ponded to later generation until all the non-additive components of variance has been mitigated to additive components.