Revealing the Intercellular Communication Changes of Human Abdominal Aortic Aneurysms at Single-cell Resolution

Abstract

Abstract Background: Abdominal aortic aneurysm (AAA) is a chronic vascular inflammation progression with a high mortality. However, intercellular communication has not been sufficiently explored in human AAA tissue. Aims: Here, we investigated available single-cell RNA sequencing data generated from human AAA. Results: These cells were divided into 27 clusters and 6 main cell types, such as T-cells, B-cells, myeloid cells, neutrophils, endothelial cells, and fibroblasts. Immune cells were present in AAA tissues more than control. In addition, we established an intercellular communication network and observed a more pronounced enrichment of TGFB1, CCL, VEGF, IL6, and complement pathways in the AAA group compared to the control group. Conclusion: Together, our analyses provide beneficial insight into cell–cell communication and expand channels for the effective immunotherapy development of AAA in the future.