Revealing the Draft Genome Sequence of Bradyrhizobium sp. Strain USDA 3458, an Effective Symbiotic Diazotroph Isolated from Cowpea (Vigna unguiculata) Genotype IT82E-16.

Richard Allen White,Jeffrey S Norman,Joseph P Dunham,Aaron Garoutte,Maren L Friesen,Emily E Mclachlan,J Cameron Thrash

doi:10.1128/mra.00813-19

Richard Allen White, Jeffrey S Norman + Show 5 more

Open Access

https://doi.org/10.1128/mra.00813-19

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Abstract

Pairing plants with plant growth-promoting bacteria is critical to the future of agriculture. Bradyrhizobium sp. strain USDA 3458 isolated from Vigna unguiculata (cowpea) paired with cowpea genotype IT82E-16 represents a novel combination in arid regions. Here, we report the draft genome sequence of strain USDA 3458.

Highlights

The rhizosphere microbiome is both numerically large and highly diverse [1,2,3]
A lyophilized culture of Bradyrhizobium sp. strain USDA 3458 was obtained from the Agricultural Research Service at the U.S Department of Agriculture (USDA) National Rhizobium Germplasm Collection
Revealing the draft genome sequence of Bradyrhizobium sp. strain USDA 3458, an effective symbiotic diazotroph isolated from cowpea (Vigna unguiculata) genotype IT82E16

Summary

Introduction

The rhizosphere microbiome is both numerically large and highly diverse [1,2,3]. Critical members of the rhizosphere microbiome are symbiotic nitrogen fixers (SNF) [4]. Strain USDA 3458 was obtained from the Agricultural Research Service at the U.S Department of Agriculture (USDA) National Rhizobium Germplasm Collection. The bacterial culture (single colony) was inoculated in AG broth [12] at 30°C at 200 rpm to obtain biomass for DNA extraction [12, 13]. DNA extraction, purification, and quality control are described in reference 13.

Results

Conclusion