Revealing the degradation mechanisms of the hyper-tolerant bacterium Pseudomonas aeruginosa STV1713 under high phenol and 2,4-DCP-induced stress conditions through RNA-seq analysis.

Abstract

The ability of bacteria to efficiently remove phenolic pollutants depends on their genetic makeup and environmental conditions. This study examined a novel strain, Pseudomonas aeruginosa STV1713, for degrading higher concentrations of phenol and 2,4-dichlorophenol. After optimization, a combination of degradation parameters, such as pH (7.0), temperature (32.5°C), and ammonium nitrate concentration (0.7g/L), was found to reduce degradation time while promoting cell growth. Under these optimal conditions, the bacterium effectively degraded up to 2000mg/L of phenol and 1400mg/L of 2,4-dichlorophenol, while maximum tolerance was observed till 2100mg/L and 1500mg/L, respectively. Metabolic profiling identified crucial metabolites in the ortho-degradation pathway during pollutant removal. Additionally, transcriptome analysis revealed that P. aeruginosa STV1713 utilizes different branches of the beta ketoadipate pathway for phenol and 2,4-DCP removal. Moreover, under high pollutant stress, the bacterium survived through differential gene expression in ribosome biogenesis, chemotaxis, membrane transport, and other pathways.