Abstract
To conduct a direct observation of cell/metal interface in microbiologically influenced corrosion (MIC), B. licheniformis cell/X80 steel interface was analyzed by combining focused-ion-beam (FIB) milling technique with scanning/transmission electron microscope (SEM/TEM). The tomography combining ion-milling with electron-imaging provided three-dimensional volumetric data about the bioinorganic interface. The spatially-resolved SEM/TEM characterization of FIB lift-out slice revealed the cellular ultrastructures thoroughly. All the results provide structural and chemical insights to MIC mechanisms and permit to a better understanding of bacteria/surface interactions.
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