Abstract

In this paper, green synthesis of iron oxide nanoparticles (IONPs) using aqueous extract of banana peel (an industrial agrowaste) and their mimetic behavior as peroxidase enzyme was investigated. The size and shape of purified nanoparticles were estimated by using transmission electron microscopy (TEM) and scanning tunneling microscopy (STM). XRD data confirm their purity and crystalline nature. Vibrating sample magnetometer (VSM) data reveals the magnetic strength of IONPs. FTIR analysis shows that biomolecules present in the banana peel extract are responsible for biosynthesis as well as stabilization of IONPs. Further, the catalytic activity of the IONPs was compared with natural horseradish peroxidase enzyme (HRP). Their comparative kinetic parameters suggest that synthesized IONPs can be used as artificial peroxidase. They can be used five times without any loss in the peroxidase mimicking activity. The synthesized nanoparticles were robust in nature as they can be used at high temperature and have a broad pH range as compared to HRP. They were further used for detection of glucose and H2O2 in solution.

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