Abstract
In this study, a microfluidic chip with integrated coil was designed and fabricated for the aim of effectively trapping magnetic nanobeads (Adembeads®, 300 nm) and measuring the chip’s temperature during the working time. In addition, a reversible technique of bonding Polydimethylsiloxane (PDMS) channels was presented. This bonding process used a coating layer of CYTOP®product as a protection, insulation and low-adhesion layer. The reversible packaging technique allows the bottom substrate to be reused, possibly equipped with sensors, and to use a disposable microchannels network. The FE method was employed to calculate the magnetic field and power consumption by the ANSYS® version 12.1 software. Merit factors were defined in order to synthetically represent the ability of the simulated coil to trap beads for a unit power consumption, i.e. a given heat generation. The simulation results propose a new approach to optimize the design criteria in fabricating planar microcoils. The optimal microcoils were fabricated and then used to realize a magnetic immunoassay in a microfluidic chip. The aim was to integrate these microcoils into a lab-on-chip and obtain a fast and highly sensitive biological element detection.
Highlights
Lab-on-a-chip devices have changed the way in which biological, medical and chemical analysis processes are being conducted by miniaturizing and automating systems
Among different ways to control beads this paper focuses on magnetic beads trapping with microcoils in microchannels on an integrated microfluidic chip
The microfluidic chip heating issues have been dealt with in different manners: in [25], an extra set of microfluidic channels have been fabricated in order to convey a cooling fluid; in [27], the microfluidic chip is operated over a Peltier thermoelectric module to regulate the chip’s temperature below 37 ◦C
Summary
Lab-on-a-chip devices have changed the way in which biological, medical and chemical analysis processes are being conducted by miniaturizing and automating systems. Bio-chips are designed to integrate a wide variety of functions such as valves, pumps, heat controller, magnetic generators, etc., which are commonly required in biological process [4,5]. The microfluidic chip heating issues have been dealt with in different manners: in [25], an extra set of microfluidic channels have been fabricated in order to convey a cooling fluid; in [27], the microfluidic chip is operated over a Peltier thermoelectric module to regulate the chip’s temperature below 37 ◦C. Despite these thermal issues, the thermal design of microcoils is scarcely studied. An advanced chip packaging technique was developed for the advantage of reusing the bottom substrates by using the conformal coating layers as a protection/insulation layer and an anti-adhesive layer on the surface of coils: CYTOP®, a Teflon-like perfluoropolymer, was previously used in [30]
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