Retrovirally Expressed Metal Response Element-Binding Transcription Factor-1 Normalizes Metallothionein-1 Gene Expression and Protects Cells against Zinc, but Not Cadmium, Toxicity

Abstract

Metal response element (MRE) transcription factor-1 (MTF1), a member of the Cys2-His2 class of zinc-finger transcription factors, is best known for its robust transcriptional regulation of mammalian metallothionein (MT) genes. MTF1 is also believed to play a generalized role in regulating genes involved in protection against heavy metals and oxidative stress. MTF1 binding to MRE motifs is regulated by changes in intracellular zinc (Zn2+) concentration. Molecular dissection of MTF1 has been hindered by its high constitutive trans-activity following transient transfection and the failure of these systems to examine genes packaged in native chromatin. In developing a system to avoid these problems, we employed a high-efficiency retroviral transduction system to reintroduce MTF1 into mouse Mtf1(−/−) knockout cells (dko7). Electrophoretic mobility shift assays demonstrated that MTF1 retrovirally transduced dko7 cells (MTF1dko7) possess levels of inducible MTF1–MRE binding activity similar to that seen in mouse hepatoma Hepa-1 cells, and MTF1 binding could be modulated over a 20-fold range by varying the concentration of Zn2+ present in the culture medium. The dko7 cells exhibited no change in Mt1 gene expression upon Zn2+ or cadmium (Cd2+) treatment; in contrast, in MTF1dko7 cells, Zn2+ or Cd2+ induced MT1 mRNA accumulation in a dose-dependent manner. Interestingly, MTF1dko7 cells showed resistance to Zn2+ toxicity, but negligible resistance to Cd2+. Concomitantly, MT1 protein levels in MTF1dko7 cells were inducible to the same degree as that in Hepa-1 cells when treated with Zn2+, but not with Cd2+. Together, our studies suggest that MTF1-mediated regulation of gene expression is sufficient to protect cells against Zn2+ toxicity and may be necessary but not sufficient to protect cells against Cd2+ toxicity.