Retraction Note: Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling

Abstract

The authors of this article have requested its retraction from Laboratory Investigation because of their inability to reproduce key experiments. The authors of this article have requested its retraction from Laboratory Investigation because of their inability to reproduce key experiments. Retraction to:Laboratory Investigation (2011) 91, 426–438; doi:10.1038/labinvest.2010.201; published online 31 January 2011 The authors of this article have requested its retraction from Laboratory Investigation because of their inability to reproduce key experiments: Zhang K, Chen D, Jiao X, Zhang S, Liu X, Cao J, Wu L, Wang D. Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodeling. Lab Invest 2011;91:426–438. This paper reports that Slug transfection does not affect E-cadherin expression. However, upon repeating the experiment, we found Slug transfection significantly reduces the E-cadherin expression. Additionally, MMP-2 was upregulated in new experiments. The paper also reveals that intracellular F-actin and MMP-9 levels are increased and relocated to the tip of the extending pseudopodia from the perinuclear pool in Slug-transfected PANC-1 cells. However, upon repeating the experiment, it appeared that only F-actin, not MMP-9, was relocated to the tip of the extending pseudopodia. Therefore, the cellular localization of the MMP-2 cells needs further investigation. Furthermore, there were several minor errors in the paper:1.The primer sequence for E-cadherin should be 5′-GGAAGTCAGTTCAGACTCCAGCC-3′ and 5′-AGGCCTTTTGACTGTAATCACACC-3′; not 5′-TTCAGTTCCGAGGTCTACAC-30; antisense: 30-GTCTCTGTGGTGATGCCGGT-5, as was reported.2.The wrong reference was cited for the BB94 treatment. The correct concentration was 0.25 μmol/l, not 0.1 umol/l, as was written.3.Female C57BL/6 mice were at 4–6 weeks, not 6–8 weeks of age.4.All statistical analyses were performed using SPSS 7.0 software, not SPSS 13. The authors regret the impact that these inconsistencies and errors may have had on other researchers. Slug enhances invasion ability of pancreatic cancer cells through upregulation of matrix metalloproteinase-9 and actin cytoskeleton remodelingLaboratory InvestigationVol. 91Issue 3PreviewSlug, a member of the Snail family of transcription factors, has a crucial role in the regulation of epithelial-mesenchymal transition (EMT) by suppressing several epithelial markers and adhesion molecules, including E-cadherin. A recent study demonstrated that no relationship exists between Slug and E-cadherin in pancreatic cancer. Another study showed that in malignant mesothelioma effusions Slug was associated with matrix metalloproteinase (MMP) expression, but that there was no association with E-cadherin. Full-Text PDF Open Access