Retraction: Hepatocyte nuclear factor-4α mediates redox sensitivity of inducible nitric-oxide synthase gene transcription

Abstract

Hepatocyte Nuclear Factor-4α Mediates Redox Sensitivity of Inducible Nitric-oxide Synthase Gene TranscriptionJournal of Biological ChemistryVol. 277Issue 7PreviewThe underlying redox-sensitive mechanisms that regulate hepatocyte expression of inducible nitric-oxide synthase (iNOS) and its antioxidant functions are largely unknown. We have demonstrated previously that oxidative stress induced by benzenetriol-mediated superoxide production increases interleukin-1β-induced iNOS protein synthesis, steady state iNOS mRNA expression, NO production, iNOS gene transcription, and trans-activation of the iNOS promoter in primary cultures of rat hepatocytes. In this study, we extend these studies by establishing the sequence specificity and binding of nuclear protein to the previously described 15-base cis-regulatory element of the rat hepatocyte iNOS promoter, isolating and identifying thecis-regulatory element transcription factor as hepatocyte nuclear factor-4α (HNF-4α), and confirming the functional role of HNF-4α in mediating redox-sensitive iNOS promoter trans-activation. Full-Text PDF Open Access VOLUME 277 (2002) PAGES 5054–5060 This article has been retracted by the publisher. The same data were reused to represent different experimental conditions. Specifically, in Fig. 1, lane 3 was reused in lane 11, and lanes 9 and 10 were reused in 17 and 18. Additionally, lane 3 from Fig. 1 was reused in lane 2 from Fig. 3. Finally, lanes 2–4 from Fig. 1 were flipped horizontally and reused in lanes 5–7 from the left panel of Fig. 4.