RETRACTED: Proteomic modulation by arsenic and microplastic toxicity in the presence of iron oxide nanoparticles in wheat (Triticum aestivum L.) seedlings

Abstract

This study provides a detailed biochemical analysis of the toxicity of arsenic (As) and polyvinyl chloride microplastics (PVC−MPs) to soil, and documents how iron oxide nanoparticles (FeO−NPs) can mitigate these effects, with a focus on the proteomic and physiological responses in wheat (Triticum aestivum L.) seedlings, expanding upon existing research in this area. For this purpose, we conducted a pot experiment in which seeds were primed with FeO−NPs at a concentration of 10 mg L−1 under toxic concentrations of As, specifically 150 and 300 mg kg−1, and PVC−MPs at concentrations of 2 and 4 mg L−1. Our results showed that the PVC−MPs and As toxicity in the soil showed a significant decline in the gas exchange attributes, sugars, AsA-GSH cycle, proline metabolism in T. aestivum seedlings. PVC−MPs and As toxicity in T. aestivum seedlings significantly increased oxidative stress biomarkers and both enzymatic and non-enzymatic antioxidants, including their gene expression, while our analysis also incorporates Profilin Quantification for a deeper understanding of the quantitative protein involvement in these processes. Although, the application of FeO−NPs showed a significant increase in the chlorophyll content, gas exchange characteristics, enzymatic and non-enzymatic compounds and their gene expression, AsA-GSH cycle and also decreased the oxidative stress. These results open new insights for sustainable agriculture practices and hold immense promise in addressing the pressing challenges of heavy metal and MPs contamination in agricultural soils.