Abstract
AbstractThe current investigation was done to examine the prevalence of Toxoplasma gondii and Neospora caninum in raw milk and traditional dairy samples. Eight‐hundred and eighty samples were collected and subjected to DNA extraction and nested PCR for detection of T. gondii B1 and N. caninum Nc5 genes. Seventy out of 880 (7.95%) and 80 out of 880 (10%) samples were positive for T. gondii and N. caninum, respectively. Simultaneous prevalence of T. gondii and N. caninum in studied samples was 4.20%. Prevalence of T. gondii and N. caninum in raw milk and traditional dairy samples were 6% and 10.60% and 10.52% and 9.21%, respectively. Traditional cheese and butter (15.00%) had the highest prevalence of T. gondii. Raw bovine (24.00%) had the highest prevalence of N. caninum. Boost prevalence of parasites in samples revealed an important public health threat regarding the consumption of raw milk and traditional dairy.Practical applicationsDue to the high consumption rate of milk and dairy products, they should have a high microbial quality. Toxoplasma gondii and Neospora caninum are two closely related protozoa with considerable zoonotic importance regarding the consumption of raw milk and traditional dairy products. The current research represents that raw milk and traditional dairy samples may be the sources of T. gondii and N. caninum transmission into the human population.
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