Abstract
Plant peroxidases belong to a multigene family and possess highly conserved domains allowing designing oligonucleotide primers to amplify DNA sequences coding for peroxidases from plants with unsequenced genomes. Peroxidase gene-based polymorphism among wild Pistacia species, and correlation between peroxidase markers were deduced in this study. Wild Pistacia germplasm was evaluated using 30 peroxidase-specific primers. Targeted-PCR amplification of genomic DNA from 100 wild accessions of Pistacia species produced polymorphisms giving a similarity range from 0.29 to 1.00 with a mean of 0.65. Based on the POGP gene polymorphism, two distinct clusters were detected among the wild accession of Pistacia species, suggesting different evolutionary pathways. Correlation estimates as an indication of linkage disequilibrium between POGP markers ranged from 0.11 to 0.90, suggesting that few POGP markers were clustered and the remaining POGP markers evenly distributed throughout the Pistacia genome. These results demonstrate that primers targeting the peroxidase gene family can be used to study genotypic diversity and evolutionary relationships on an intra- and inter-specific basis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
