RETRACTED ARTICLE: LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration

Qing-An-Zi Wang,Yongxiu Yang,Xiaolei Liang

doi:10.1186/s13048-020-00639-2

Qing-An-Zi Wang, Yongxiu Yang + Show 1 more

Open Access

https://doi.org/10.1186/s13048-020-00639-2

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Abstract

BackgroundAlthough lncRNA CTBP1-AS2 has been functionally analyzed only in cardiomyocyte hypertrophy and diabetes, analysis of TCGA dataset revealed its downregulation in endometrial carcinoma (EC), indicating its involvement in EC.ResultsIn this study we found that CTBP1-AS2 was downregulated in EC and correlated with poor survival. MiR-216a might form base pairs with CTBP1-AS2 based on RNA-RNA interaction, which was confirmed by luciferase activity assay. Interestingly, upregulation of PTEN was observed after CTBP1-AS2 overexpression. Transwell assay showed that CTBP1-AS2 and PTEN overexpression led to decreased cancer cell invasion and migration and reduced enhancing effects of miR-216a on cell invasion and migration. It was known that miR-216a targeted PTEN.ConclusionTherefore, CTBP1-AS2 may sponge miR-216a to upregulate PTEN, thereby suppressing endometrial cancer cell invasion and migration.

Highlights

CTBP1-AS2 was downregulated in Endometrial carcinoma (EC) and correlated with combination of CTBP1-AS2 vector+miR-216a or the combination of CTBP1-AS2 vector+negative control (NC) miRNA (NC group)
E relatively luciferase activity was significantly lower in miR-216a group than in NC group (Fig. 2b, p < 0.05)
C HEC-1 cells were transfected with CTBP1-AS2 expresI sion vector or miR-216a mimic to analyze the interactions between CTBP1-AS2 and miR-216a

Summary

Conclusion

CTBP1-AS2 may sponge miR-216a to upregulate PTEN, thereby suppressing endometrial cancer cell invasion and migration. Based on the clinical symptoms and risk factors, early detection of EC is possible in some. Functional analysis of molecular regulators in EC would facilitate the development of novel anti-EC approaches, such as targeted therapies, which aim to suppress cancer development by regulating cancer-related gene expression [8, 9]. Long non-coding RNAs (lncRNAs) are not involved in protein synthesis but can regulate gene expression at multiple levels to participate in human diseases, including cancers [10]. LncRNAs interact with cancer-related proteins and other non-coding RNAs, such as miRNAs [11]. CTBP1-AS2 has been characterized as a critical player in type 2 diabetes and cardiomyocyte hypertrophy [12, 13]. Gansu Province 730000, PR China the expression of CTBP1-AS2 by TCGA dataset, and.

Methods

Results

E Availability of data and materials

E References