RETRACTED ARTICLE: Gene expression analysis in response to osmotic stimuli in the intervertebral disc with DNA microarray

Wenzhi Zhang,Yefeng Hu,Liqun Duan,Qichun Zhao,Rui He,Feng Zhang,Xiang Xu,Xifu Shang,Xu Li

doi:10.1186/2047-783x-18-62

Abstract

BackgroundIntervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment. At present, the molecular mechanisms underlying osmotic regulation in IVD cells are poorly understood. This study aims to screen genes affected by changes in osmotic pressure in cells of subjects aged 29 to 63 years old, with top-scoring pair (TSP) method.MethodsGene expression data set GSE1648 was downloaded from Gene Expression Omnibus database, including four hyper-osmotic stimuli samples, four iso-osmotic stimuli samples, and three hypo-osmotic stimuli samples. A novel, simple method, referred to as the TSP, was used in this study. Through this method, there was no need to perform data normalization and transformation before data analysis.ResultsA total of five pairs of genes ((CYP2A6, FNTB), (PRPF8, TARDBP), (RPS5, OAZ1), (SLC25A3, NPM1) and (CBX3, SRSF9)) were selected based on the TSP method. We inferred that all these genes might play important roles in response to osmotic stimuli and age in IVD cells. Additionally, hyper-osmotic and iso-osmotic stimuli conditions were adverse factors for IVD cells.ConclusionsWe anticipate that our results will provide new thoughts and methods for the study of IVD disease.

Highlights

Intervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment
Osmotic pressure and related genes Based on the top-scoring pair (TSP) method, a total of 65 gene pairs were selected from the three groups, including 34 gene pairs in the hyper-osmotic vs. hypo-osmotic group (Table 1), 7 in hyper-osmotic vs. iso-osmotic group (Table 2), and 24 in iso-osmotic vs. hypo-osmotic group (Table 3)
In the hyper-osmotic stimuli samples, the gene expression value of CBX3 was more than that of serine/arginine rich splicing factor 9 (SRSF9) at the age of 29 and the situation was reversed at the age of 50–63. All these results indicated that the expression level of these gene pairs ((CYP2A6, FNTB), (PRPF8, TARDBP), (RPS5, OAZ1), (SLC25A3, NPM1) and (CBX3, SRSF9)) at different osmotic pressures did not change at an early age, but was reversed in old age

Summary

Introduction

Intervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment. Intervertebral disc (IVD) disease is a frequent surgical disease characterized by a series of deleterious changes in cellularity that lead to loss of extracellular matrix structure, altered biomechanical loading, and symptomatic pain [1]. Studies of biological therapeutics for IVD disease have been ongoing over a decade, few treatments have progressed to clinical testing and none is current commercially available [2,3,4]. This may be, primarily, due to a limited understanding of disease etiology. The IVD cells can secrete a complex extracellular matrix containing

Objectives

Methods

Results

Discussion

Conclusion