Abstract
BackgroundOver the years, long non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on the role of lncRNA cartilage injury-related (lncRNA-CIR) in osteosarcoma cells.MethodsLncRNA-CIR expression in osteosarcoma tissues and cells, and adjacent normal tissues and normal osteoblasts was determined, then the relations between lncRNA-CIR expression and the clinicopathological features, and between lncRNA-CIR expression and the prognosis of osteosarcoma patients were analyzed. Moreover, the MG63 and 143B cells were treated with silenced or overexpressed lncRNA-CIR, and then the proliferation, invasion, migration and apoptosis of the cells were evaluated by gain- and loss-of-function approaches. The tumor growth, and proliferation and apoptosis of osteosarcoma cells in vivo were observed by subcutaneous tumorigenesis in nude mice.ResultsWe have found that lncRNA-CIR was up-regulated in osteosarcoma tissues and cells, which was respectively relative to adjacent normal tissues and normal osteoblasts. The expression of lncRNA-CIR was evidently correlated with disease stages, distant metastasis and differentiation of osteosarcoma patients, and the high expression of lncRNA-CIR indicated a poor prognosis. Furthermore, the reduction of lncRNA-CIR could restrict proliferation, invasion and migration, but promote apoptosis of osteosarcoma cells in vitro. Meanwhile, inhibited lncRNA-CIR also restrained tumor growth and osteosarcoma cell proliferation, whereas accelerated apoptosis of osteosarcoma cells in vivo.ConclusionWe have found in this study that the inhibited lncRNA-CIR could decelerate proliferation, invasion and migration, but accelerate apoptosis of osteosarcoma cells, which may provide a novel target for osteosarcoma treatment.
Highlights
IntroductionLong non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on the role of long non-coding RNAs (lncRNAs) cartilage injury-related (lncRNA-CIR) in osteosarcoma cells
Over the years, long non-coding RNAs have been clarified in malignancies, this research was focused on the role of lncRNA cartilage injury-related in osteosarcoma cells
LncRNA‐CIR is up‐regulated in osteosarcoma tissues and cells, and the highly‐expressed lncRNA‐CIR indicates a poor prognosis of osteosarcoma patients To determine the expression of lncRNA-CIR in osteosarcoma, reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to measure lncRNA-CIR expression in osteosarcoma tissues and cell lines
Summary
Long non-coding RNAs (lncRNAs) have been clarified in malignancies, this research was focused on the role of lncRNA cartilage injury-related (lncRNA-CIR) in osteosarcoma cells. Liu et al [9] identified that lncRNA cartilage injury-related (lncRNA-CIR) may increase the degradation of chondrocyte extracellular matrix in osteoarthritis (OA). LncRNA-CIR has been proved to be up-regulated in bladder cancer tissues and cell lines [12] Based on these data, we speculate that lncRNA-CIR may be associated with bone diseases and overexpressed in cancer tissues. To further unravel the effect of lncRNA-CIR on human diseases, we conducted this research to explore the role of lncRNA-CIR in osteosarcoma development, and we speculate that the function of lncRNA-CIR may be related to the malignant phenotypes of osteosarcoma cells in vitro and osteosarcoma tumor growth in vivo
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