RETRACTED ARTICLE: Curcumin inhibits proliferation, migration, invasion and promotes apoptosis of retinoblastoma cell lines through modulation of miR-99a and JAK/STAT pathway

Yaping Li,Ning Han,Weixuan Sun,Dexin Yin,Ying Zou

doi:10.1186/s12885-018-5130-y

Abstract

BackgroundCurcumin, a primary active ingredient extracted from the Curcuma longa, has been recently identified as a potential anti-tumor agent in multiple kinds of cancers. However, the effect of curcumin on retinoblastoma (Rb) is still unclear. Therefore, we attempted to reveal the functional impacts and the underlying mechanisms of curcumin in Rb cells.MethodsTwo Rb cell lines SO-Rb50 and Y79 were pre-treated with various doses of curcumin, and then cell proliferation, apoptosis, migration, and invasion were assessed, respectively. Further, regulatory effects of curcumin on miR-99a expression, as well as the activation of JAK/STAT pathway were studied.ResultsData showed that curcumin significantly inhibited the viability, colony formation capacity, migration and invasion, while induced apoptosis of SO-Rb50 and Y79 cells. Up-regulation of miR-99a was observed in curcumin-treated cells. Curcumin suppressed the phosphorylation levels of JAK1, STAT1, and STAT3, while curcumin did not inhibit the activation of JAK/STAT pathway when miR-99a was knocked down.ConclusionCurcumin inhibited proliferation, migration, invasion, but promoted apoptosis of Rb cells. The anti-tumor activities of curcumin on Rb cells appeared to be via up-regulation of miR-99a, and thereby inhibition of JAK/STAT pathway.

Highlights

Curcumin, a primary active ingredient extracted from the Curcuma longa, has been recently identified as a potential anti-tumor agent in multiple kinds of cancers
In vitro investigation has showed that curcumin inhibited bladder cancer cells growth and metastasis via regulating β-catenin
Curcumin inhibited the proliferation of SO-Rb50 and Y79 cells To start with, SO-Rb50 and Y79 cells were subjected with 0–50 μM of curcumin, and the viability of cell was tested after 24 h treatment with curcumin

Summary

Introduction

A primary active ingredient extracted from the Curcuma longa, has been recently identified as a potential anti-tumor agent in multiple kinds of cancers. The effect of curcumin on retinoblastoma (Rb) is still unclear. The overall survival rates have been significantly improved recently, the survival rate of Rb in the Curcumin, a nature yellow pigment, is the primary active ingredient extracted from the rhizome of the East Indian plant Curcuma longa [5]. Multiple therapeutic properties of curcumin have been recognized, including anti-inflammatory, anti-neoplastic [6], anti-oxidant [7], anti-fibrotic [8], and anti-ischemic [9] effects. Regarding the anti-neoplastic function, curcumin has been reported to suppress the secretion of gastrin-mediated acid, and thereby inhibiting the progression of gastric cancer [10]. In vitro investigation has showed that curcumin inhibited bladder cancer cells growth and metastasis via regulating β-catenin

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