Abstract
Background/aimsThe dysregulation of circABCB10 may play an critical role in tumorprogression. However, its function in liver cancer (HCC) is still unclear.Therefore, this experimental design is based on circABCB10 to explore thepathogenesis of HCC.MethodsThe expression of circABCB10 and miR-670-3p in HCC tissues wasdetected by RT-qPCR. CCK-8, Brdu incorporation, colony formation and transwellassays were used to determine the effect of circABCB10 on HCC cell proliferationand migration. Target gene prediction and screening, luciferase reporter assayswere used to validate downstream target genes of circABCB10 and miR-670-3p.HMG20A expression was detected by RT-qPCR and Western blotting. The tumorchanges in mice were detected by in nude mice.ResultsCircABCB10 was significantly increased in HCC tissues and celllines, and high CircABCB10 expression was directly associated with low survivalin HCC patients. Silencing of circABCB10 inhibited proliferation and invasion ofhepatocellular carcinoma. In addition, circABCB10 acted as a sponge ofmiR-670-3p to upregulate HMG20A expression. In addition, overexpression ofmiR-670-3p or knockdown of HMG20A reversed the carcinogenic effects ofcircABCB10 in HCC. There was a negative correlation between the expression ofcircABCB10 and miR-670-3p, and a positive correlation between the expression ofcircABCB10 and HMG20A in HCC tissues.ConclusioncircABCB10 promoted HCC progression by modulating themiR-670-3p/HMG20A axis, and circABCB10 may be a potential therapeutic target forHCC.Trail registration JL1H384739,registered at Sep 09, 2014.
Highlights
Primary liver cancer is the third leading cause of cancerrelated death [1, 2]
Circ circular RNA (RNA) has been reported to be involved in multiple processes of tumor progression, which provides a new direction for cancer therapy [8, 9]
Statistical analysis of TCGA data from UALCAN indicated that the expression of circABCB10 was obviously higher than that in normal liver tissues (P < 0.001, Fig. 1b)
Summary
Primary liver cancer is the third leading cause of cancerrelated death [1, 2]. The most common type of primary liver cancer is hepatocellular carcinoma (HCC) [3]. Circ RNA has been reported to be involved in multiple processes of tumor progression, which provides a new direction for cancer therapy [8, 9]. There is increasing evidence that circ RNA is involved in autophagy-related, apoptosis, cell cycle and proliferation, regulating tumor cell growth, apoptosis and cell cycle progression, suggesting that circ RNA may be a novel therapeutic target in cancer [10, 11]. Circ ABCB10 is highly expressed in embryonic tissues and is not expressed in most adult tissues It is highly expressed in tumor tissues such as breast cancer [13]. There is a lack of literature on the regulation of liver cancer by circABCB10
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