Abstract
BackgroundAdiponectin, a protein hormone produced by adipose tissues, exhibits anti-inflammatory functions in various models. This study was investigated the effects of adiponectin on dextran sodium sulfate (DSS)-colonic injury, inflammation, apoptosis, and intestinal barrier dysfunction in Caco-2 cell and mice.Materials and methodsThe results showed that DSS caused inflammatory response and intestinal barrier dysfunction in vitro and in vivo. Adiponectin injection alleviated colonic injury and rectal bleeding in mice. Meanwhile, adiponectin downregulated colonic IL-1β and TNF-α expressions and regulated apoptosis relative genes to attenuate DSS-induced colonic inflammation and apoptosis. Adiponectin markedly reduced serum lipopolysaccharide concentration, a biomarker for intestinal integrity, and enhanced colonic expression of tight junctions (ZO-1 and occludin). The in vitro data further demonstrated that adiponectin alleviated DSS-induced proinflammatory cytokines production and the increased permeability in Caco-2 cells.ConclusionAdiponectin plays a beneficial role in DSS-induced inflammation via alleviating apoptosis and improving intestinal barrier integrity.
Highlights
Adiponectin, a protein hormone produced by adipose tissues, exhibits anti-inflammatory functions in various models
Compelling evidence has demonstrated the anti-inflammatory function of adiponectin in various inflammatory models, such as skin inflammation [12], peripheral inflammation [13], LPS-induced inflammatory response in adipocytes [14]
From days 4 to 10, dextran sodium sulfate (DSS) exposure significantly increased rectal bleeding score and diarrhea score compared with the control group (P < 0.05)
Summary
Adiponectin, a protein hormone produced by adipose tissues, exhibits anti-inflammatory functions in various models. This study was investigated the effects of adiponectin on dextran sodium sulfate (DSS)-colonic injury, inflammation, apoptosis, and intestinal barrier dysfunction in Caco-2 cell and mice. Adiponectin injection alleviated colonic injury and rectal bleeding in mice. The in vitro data further demonstrated that adiponectin alleviated DSS-induced proinflammatory cytokines production and the increased permeability in Caco-2 cells. Conclusion Adiponectin plays a beneficial role in DSS-induced inflammation via alleviating apoptosis and improving intestinal barrier integrity. Compelling evidence has demonstrated the anti-inflammatory function of adiponectin in various inflammatory models, such as skin inflammation [12], peripheral inflammation [13], LPS-induced inflammatory response in adipocytes [14]. In this study, effects of adiponectin on dextran sodium sulfate (DSS)-colonic inflammation and injury were investigated in Caco-2 cell lines and Kunming mice
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
