Abstract
This chapter focuses on procedures that can be employed in the characterization of the role of cellular retinoic acid-binding protein (CRABP) and cellular retinol-binding protein (CRBP) in retinoid-related cellular behavior. Embryonal carcinoma (EC) cells are described as prototypes for such studies because they, like many other cell types, possess CRABP, CRBP, and retinoic acid receptors (RAR). Embryonal carcinoma cells differentiate readily in response to retinoids, an event that can be monitored in a number of convenient ways. Furthermore, EC lines can be induced to metabolize retinoids, a phenomenon that might involve the retinoid-binding proteins. The chapter examines methods to characterize CRABP and CRBP protein and RNA levels. It also outlines strategies for producing mutant cell lines that lack detectable CRABP activity.
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