Retinoic Acid Treatment of Human Hematological Malignancies Induces Caspase Dependent and Independent Apoptotic Cell Death

Abstract

The unprejudiced of this education is to gauge the ability of the retinoic acid to induce apoptotic cell deathin hematological tumors through caspase dependent or independent apoptotic pathway, The cytotoxicityeffects of retinoic acid of different concentrations (400,350,300,250,200,150,100,50,25,12.5 µg\ml) andexposure for all hematological malignancy cell lines (Human non-Hodgkin lymphoma SR and humanmultiple myeloma (COLO 677) and Human Monocytic Leukemia THP1 and Acute promyelocytic leukemiaNB4) have been determined using a microtetrazolium (MTT) assay. Propodeum iodide and alcidine orange(AO/PI) paired discoloration was used to study the ability of retinoic acid to induce apoptosis in the infectedcells and examined under fluorescence microscope and quantified for the percentage of apoptosis induction.Quantitative immunocytochemistry assay was used to study the caspase dependent and independentproteins expression in infected and control cells. Cells treated with Retinoic Acid showed increased celldeath percentage compared to the untreated cells as quantified by MTT assay. AO/PI results revealed thatRetinoic Acid had powerful effect on inducing apoptosis significantly (p<0.001) in human cancer cell linestested, compared to control cell. Immunocytochemistry in Retinoic Acid infected human hematological celllines revealed remarkable increase in expression of caspase 8,9 (dependent pathway) and AIF, ENDOG(independent pathway) induces a significant (p<0.002) as compared untreated cell.This study, which shows the role of the Retinoic Acid in inducing apoptosis through a dependent andindependent pathway in cancer cells, we anticipation these annotations will shanty light on the impendingexploration of retinoic acid in cancer hindrance and rehabilitation