Retinoic acid signaling in heart development: Application in the differentiation of cardiovascular lineages from human pluripotent stem cells.

Alexandra Wiesinger,Vincent M Christoffels,Gerard J.J Boink,Harsha D Devalla

doi:10.1016/j.stemcr.2021.09.010

Alexandra Wiesinger, Vincent M Christoffels + Show 2 more

Open Access

https://doi.org/10.1016/j.stemcr.2021.09.010

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Journal: Stem cell reports	Publication Date: Oct 14, 2021
Citations: 29	License type: cc-by

Affiliation: Amsterdam University Medical Centers

Abstract

SummaryRetinoic acid (RA) signaling plays an important role during heart development in establishing anteroposterior polarity, formation of inflow and outflow tract progenitors, and growth of the ventricular compact wall. RA is also utilized as a key ingredient in protocols designed for generating cardiac cell types from pluripotent stem cells (PSCs). This review discusses the role of RA in cardiogenesis, currently available protocols that employ RA for differentiation of various cardiovascular lineages, and plausible transcriptional mechanisms underlying this fate specification. These insights will inform further development of desired cardiac cell types from human PSCs and their application in preclinical and clinical research.

Highlights

Retinoic acid (RA), the active derivative of vitamin A, has pleiotropic functions during vertebrate development, differentiation, and homeostasis
We focus on the role of RA in heart development, in the specification of various cardiac cell types and its application in differentiating these cell populations from human pluripotent stem cells
RA is utilized in the differentiation of multiple cardiac cell types from human pluripotent stem cells (hPSCs) (Figure 7)

Summary

SUMMARY

Retinoic acid (RA) signaling plays an important role during heart development in establishing anteroposterior polarity, formation of inflow and outflow tract progenitors, and growth of the ventricular compact wall. RA is utilized as a key ingredient in protocols designed for generating cardiac cell types from pluripotent stem cells (PSCs). This review discusses the role of RA in cardiogenesis, currently available protocols that employ RA for differentiation of various cardiovascular lineages, and plausible transcriptional mechanisms underlying this fate specification. These insights will inform further development of desired cardiac cell types from human PSCs and their application in preclinical and clinical research

INTRODUCTION

D15 SANCMs

Findings

CONCLUDING PERSPECTIVES