Abstract
BackgroundDespite endometriosis is common estrogen dependent disease afflicting women in reproductive age, the pathogenesis has not been fully elucidated. Retinoic acid has various functions in cells as biologic modulator, and aberrant retinoid metabolism seems to be involved in the lesions of endometriosis. In order to evaluate the potential of all-trans retinoic acid (ATRA) for therapeutic treatment, a transcriptome analysis and estradiol measurements in cultured endometriotic cells and tissues were conducted.MethodsThe mRNA expression levels in ATRA-treated endometriotic stromal cells (ESC) isolated from ovarian endometrial cysts (OEC) were investigated. Estradiol production in OEC tissues was also investigated.ResultsIn the isolated ESC culture supplemented with ATRA for four days, total RNA was extracted followed by a transcriptome analysis using GeneChip. Forty-nine genes were upregulated and four genes were down-regulated by the ATRA treatment. Many upregulated genes were associated with the negative regulation of cellular proliferation. In addition, ATRA treatment decreased the mRNA expression of 17-beta-dehydrogenase 2 (HSD17B2) which converts estradiol into estrone in a dose-dependent manner, and the ELISA measurements indicated that estradiol production in the OEC tissue was inhibited by ATRA treatment.ConclusionsRetinoic acid has the potential to suppress endometriosis development.
Highlights
Endometriosis is a common gynecological disease affecting approximately 10 % of reproductive age females
We examined the effects of all-trans retinoic acid (ATRA) on the gene expression in isolated and cultured choESC
Quantitative RT-PCR Among the genes differentially expressed in the ATRAtreated choESC compared to the control cells, we focused on the IRF8, TNFSF13B, WNT4, RARRES1, IGFBP3, PSMB9, RARRES3, IGFBP6, CYP26B1, IDO1 and RARE genes associated with negative cellular proliferation
Summary
Endometriosis is a common gynecological disease affecting approximately 10 % of reproductive age females. This condition is characterized by the ectopic localization of endometrial-like tissue in the pelvic cavity. Despite endometriosis is common estrogen dependent disease afflicting women in reproductive age, the pathogenesis has not been fully elucidated. Retinoic acid has various functions in cells as biologic modulator, and aberrant retinoid metabolism seems to be involved in the lesions of endometriosis. In order to evaluate the potential of all-trans retinoic acid (ATRA) for therapeutic treatment, a transcriptome analysis and estradiol measurements in cultured endometriotic cells and tissues were conducted
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