Abstract
RAW264 macrophage-like cells contain a kinase which is dependent on Ca ++ and phosphatidylserine for activity (C kinase) and is stimulable by the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate. Retinal inhibits the activity of the tumor promoter-activated kinase in a concentration-dependent manner. The apparent Ki for inhibition is 1 × 10 −5M. Retinal is not a general inhibitor of phosphotrans-ferase reactions as it did not inhibit the activity of purified cyclic AMP-dependent protein kinase. It is possible, therefore, that the action of retinoids to antagonize tumor promoter effects on cell function may be mediated at the level of regulation of C-kinase.
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