Retinal characterization in the eyes of two bats endemic in the Egyptian fauna, the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrellus kuhlii), using the light microscope and transmission electron microscope

Abstract

Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrellus kuhlii). Seven eyes were collected from adult individuals of each species. Examination of the retina using a light microscope and a transmission electron microscope was carried out. The retina of P. kuhlii was thicker than that of R. aegyptiacus, which had many projections extended from the choroid layer into retina forming papillae. Despite rods being dominant in retinae of both species, cone photoreceptors were encountered in both retinae. The outer plexiform layer of R. aegyptiacus was arranged into islets between the outer nuclear layer produced differences in its thickness. However, the retina of P. kuhlii showed a normal arrangement of retinal structure. The retinal pigment epithelium of both bat species consists of a single layer of the cuboidal cells with a round to oval vesicular nuclei, which showed a lack of pigmentation in R. aegyptiacus and poor pigmentation in the P. kuhlii. In conclusion, our investigation detected many structural and ultrastructural differences between the two bat species. The presence of many projections protruded from the choroid layer of R. aegyptiacus retina is considered the most characteristic difference between the retinae of R. aegyptiacus and P. kuhlii.