Abstract
The understanding of retention processes in supercritical fluid chromatography is important for the continued growth and development of the analytical technique. Chromatographic retention has been studied using a simple thermodynamic model involving solute retention as a function of pressure and through assessment of the effect of density on the enthalpy of solute transfer between the supercritical mobile phase and bonded stationary phase. The solvatochromic behavior of solute probes in pure and binary supercritical fluids can be used to determine their polarity/polarizability as a function of density and related solvent effects on solute retention in supercritical fluid chromatography.
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