Abstract
As part of a study to predict the retention of analytes in reversed-phase liquid chromatography the effects of isomerisation and unsaturation in alkyl chain substituents on an aromatic ring have been examined. The contributions of the presence of branching and of olefinic groups have been related to the eluent composition in methanol-buffer and acetonitrile-buffer eluents. These results have been applied to a comparison of the influence on retention of primary, secondary and tertiary hydroxyl groups in isomeric phenylpropanols.
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