Abstract
Abstract Sedimentation field-flow fractionation (SFFF) is a promising method for the high-resolution separation of a wide variety of suspended particulates and dissolved macromolecules. By using a new SFFF technique with a time-delayed exponential force-field (rotor speed) decay, quantitative particle-size distribution analyses in the 0.01–1 μm range can be performed in a few minutes. Relative to constant-field SFFF, programmed force-field operation can drastically decrease analysis time and improve detection sensitivity while maintaining adequate resolution. The linear relationship between particle retention and logarithm of particle diameter or mass for the new technique significantly simplifies data handling for convenient and accurate analyses. Standard graphs have been prepared to show how separation variables such as exponential decay time constant, τ, initial rotor speed, ωo, channel thickness, W, and flowrate, F, can affect particle retention. These simple and quantitative relationships are useful...
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