Abstract
Abstract The reversed-phase liquid chromatographic retention behavior of poly(l-tryptophan)s and poly(d,l-tryptophan)s whose molecular weight ranged from 5.4 kD to 37.25 kD is examined using a C-8 chemically bonded stationary phase and binary mobile phases of tetrahydrofuran (THF)-water and ternary mobile phases of THF-water-methanol. The retention of poly(l-tryptophan)s is compared with that of poly(d,l-tryptophan)s. Linear-Solvent-Strength (LSS) model is examined in describing the retention behavior. The effect of adding a third solvent to the binary mobile phase is also discussed.
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