Abstract
The reversed-phase chromatographic behaviour of a homologous series has been investigated as a function of the mobile phase composition and temperature. In general, it was found that the methylene selectivity decreased with increasing temperature and decreasing amount of water in the mobile phase. The dependence of the calculated data on the correct determination of the hold-up time is discussed. The methylene group contribution to Δ H of transfer was calculated using two different methods and the agreement was excellent. In addition, the agreement with one other reported value was excellent. Using the homologous series data, the solvent strength of the water-methanol mobile phases was calculated at different temperatures. The results were compared with the data obtained on pyrocarbon supports and the agreement was very good. Linearization methods for calculating t o are discussed and the difficulties in using such procedures are described.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
