Abstract
Many columns for reversed phase HPLC, based on silica gel, frequently used for separation of natural antioxidants, have a limited thermal and pH stability. In contrast, zirconium dioxide based stationary phases have an extremely different surface chemistry and are chemically stable over a wide rage of pH (1 to 14). Chromatographic properties associated with attractive and repulsive ionic interactions were investigated by measuring the retention data of isomeric naphthalene disulphonic acids on three zirconia based columns with carbon, carbon C18, and polystyrene bonded stationary phases. A comparative study on the retention behavior of natural antioxidants on zirconia based stationary phases is reported. The results suggest that zirconia based columns could be successfully used for separation of natural phenolic antioxidants.
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