Resveratrol-dependent sensitization of prostate cancer cells (165.33)

Abstract

Abstract Prostate Cancer (PCa) is the most common non-skin malignancy and commonly diagnosed cancer, and second most cancer responsible for death in men in United States. Prior studies in animal models of PCa as well as in human, demonstrated that the prostate produces several growth factors. Addition or blocking of these growth factors can alter the PCa cell proliferation and other important functions. Resveratrol (RES), a polyphenolic compound found in the skin of red fruits, exhibits anti-inflammatory, anti-oxidative, anti-cancer, and anti-proliferative characteristics. In this study, the role RES has been investigated on mouse prostate cancer (PCa) cell lines, derived from transgenic adenocarcinoma of mouse prostate (TRAMP), in C57/B6 mice. Interestingly, TRAMP-C1 and TRAMP-C2 form tumors while TRAMP-C3 fails to form tumors. TRAMP cells were treated with different concentration of resveratrol and at different time points analyzed for intensity of sensitization, cytokines, growth factors as well as apoptotic genes. The results show that TRAMP C1 and C2 were resistant to RES compared to TRAMP C3 suggesting that TRAMP-C3 cells may up regulate “kill me signal” that help in sensitization of these cells compared to TRAMP C1 and C2. This data also demonstrates that RES mode of action is specific for each cell line in activating program cell death that may utilize unique sets of genes during apoptotic pathways.