Resveratrol treatment improves plasma and blood glucose concentration and lipid metabolism in high-fat-fed C57BL/6J mice

Abstract

SIRT1 is the homologue of SIR2 that links to longevity in lower organisms and has a direct or indirect role that positively regulates the metabolic pathway via insulin signaling. RSV, a naturally occurring small polyphenol that activates SIRT1, has been reported to ameliorate the early development of metabolic syndrome of high-fat-diet-fed mice at a low dose (0.005 w/w) in 10 weeks through inhibition of fatty acid synthase and other relevant enzymes. However, what effect of a higher dose of dietary RSV on the long-term high-fat-diet-fed C57BL/6J mice has not been reported in view of regulating SIRT1 and related genes. This study investigated the effect of a higher dose of RSV (0.1 % w/w) on glucose and lipid metabolism in 13-week high-fat-diet-fed C57BL/6J mice as well as on mRNA and protein expression regulations with respect to relevant genes. The data showed that a higher dose of RSV supplementation significantly lowered the concentration of glucose, plasma total cholesterol, total triglyceride concentrations, and hepatic cholesterol in high-fat-diet-fed mice. Relative mRNA expressions of SIRT1, FOXO1, PPARγ, and PGC-1α were significantly up-regulated, and corresponding protein expressions were also enhanced.