Resveratrol suppresses type 2 cytokine induction in basophilic mast cell and Th2 cell infiltration in PCA mice

Abstract

Cytokines released from mast cells, may play roles to initiate and maintain allergic responses. The high amount of cytokines accumulation can excite the late‐phase allergic responses, and it happens in consistence with migration of other leukocytes including neutrophils, lymphocytes, eosinophils and macrophages. Therefore, this study was conducted to test the resveratrol effects on IgE‐mediated basophilic mast cell and passive cutaneous anaphylaxis (PCA) in mice <em style=”mso‐bidi‐font‐style: normal;”>via cytokine suppression. Basophilic mast cells were sensitized with anti‐dinitrophenyl‐immunoglobulin E (anti‐DNP‐Ig E) and then pre‐treated with 1‐25 uM resveratrol depending on treatments. The DNP‐human serum albumin antigen was subsequently stimulated and all cells were then sampled for analysis. Similar <em style=”mso‐bidi‐font‐style: normal;”>in vivo experiment was conducted due to oral administration of 10 mg/kg resveratrol to BALB/c mouse (n = 5). Resveratrol diminished the secretion of interleukin (IL)‐3, IL‐4, IL‐13 and tumor necrosis factor(TNF)‐α in anti‐DNP‐IgE‐sensitized cells. Oral administration of 10 mg/kg resveratrol also reduced serum IL‐3, IL‐4, IL‐13 and TNF‐α level, on the contrary high level of IL‐3, ‐4 and ‐13 and TNF‐a were determined when BALB/c were sensitized and then challenged to DNP‐HSA. All DNP‐HSA mouse had high Tyrosine kinase (Tyk) 2 and Signal Transducer and Activator of Transcription (STAT). Resveratrol alleviated T cell infiltration of ear and dorsal skin in mice. In summary, the results indicated that the resveratrol administration lightened Type 2 cytokine releases and then alleviated the cytokine‐mediated allergic responses.