Resveratrol protects oxygen-glucose deprivation rat cortical neurons by regulating SIRT1/AMPK signaling pathways

Abstract

Objective To investigate the effects of resveratrol on the first and double oxygen-glucose deprivation (OGD) primary cortical neuron silent information regulator 1 (SIRT1), AMP-activated protein kinase (AMPK) activity and ATP content, and its possible neuroprotective mechanism. Methods Cortical neurons were taken from the embryos of 18-day Wistar rats. An in vitro repeated ischemia model was induced by the double OGD after the success of primary culture. Trypan blue staining was used to detect the cell survival rate. Western blot was used to detect the SIRT1 and phospho-AMPK expression. Deacetylase fluorescence assay was used to detect the SIRT1 activity. Bioluminescence assay was used to detect the ATP content. Results Compared with the control group, resveratrol (0.5 μmol/L) preconditioning significantly increased the survival rates after the single and double OGD (all P<0.001), ATP content (all P=0.004), SIRT1 activity (single: P=0.001; double: P=0.002), and the expression levels of SIRT1 (single: P=0.029; double: P=0.023) and phospho-AMPK (all P=0.001). Conclusions Resveratrol has the neuroprotective effect for the first and double OGD cortical neurons. Its mechanism may be associated with upregulating the SIRT1/AMPK signaling pathways and decreasing the energy requirements. Key words: Neurons; Cells, Cultured; Oxygen; Glucose; Stilbenes; Sirtuin 1; AMP-Activated Protein Kinases; Neuroprotective Agents; Rats; Resveratrol