Abstract
Resveratrol, a polyphenol presents in grapes and wine, displays antioxidant and anti-inflammatory properties and cytoprotective effect in brain pathologies associated to oxidative stress and neurodegeneration. In previous work, we demonstrated that resveratrol exerts neuroglial modulation, improving glial functions, mainly related to glutamate metabolism. Astrocytes are a major class of glial cells and regulate neurotransmitter systems, synaptic processing, energy metabolism and defense against oxidative stress. This study sought to determine the protective effect of resveratrol against hydrogen peroxide (H2O2)-induced cytotoxicity in C6 astrocyte cell line, an astrocytic lineage, on neurochemical parameters and their cellular and biochemical mechanisms. H2O2 exposure increased oxidative-nitrosative stress, iNOS expression, cytokine proinflammatory release (TNFα levels) and mitochondrial membrane potential dysfunction and decreased antioxidant defenses, such as SOD, CAT and creatine kinase activity. Resveratrol strongly prevented C6 cells from H2O2-induced toxicity by modulating glial, oxidative and inflammatory responses. Resveratrol per se increased heme oxygenase 1 (HO1) expression and extracellular GSH content. In addition, HO1 signaling pathway is involved in the protective effect of resveratrol against H2O2-induced oxidative damage in astroglial cells. Taken together, these results show that resveratrol represents an important mechanism for protection of glial cells against oxidative stress.
Highlights
The polyphenol resveratrol (3,5,49-trihydroxy-trans-stilbene), a redox active compound, is a phytoalexin found in a wide variety of dietary sources including grapes, peanuts and wines, especially red wines [1,2,3,4,5]
We examined the effect of heme oxygenase 1 (HO1) inhibitor on extracellular GSH levels, incubating Zinc Protoporphyrin IX (ZnPP IX) 10 mM, for 0.5 h before the treatment described for resveratrol and H2O2
To test whether decreased in nitrite accumulation induced by resveratrol was dependent on NOS activity, we examined the effect of resveratrol in the presence of the L-NAME (Nv-nitro-L-arginine methyl ester), a NOS inhibitor
Summary
The polyphenol resveratrol (3,5,49-trihydroxy-trans-stilbene), a redox active compound, is a phytoalexin found in a wide variety of dietary sources including grapes, peanuts and wines, especially red wines [1,2,3,4,5]. Resveratrol presents important antioxidant properties, possibly by its direct scavenging effect and/or activation of pathways those upregulate natural antioxidant defenses [6,7]. Many studies have shown that resveratrol can prevent or slow the progression of a wide variety of illnesses, including cancer [8] and cardiovascular diseases [9,10] and improves health and survival of mice on a high-calorie diet [11]. It has been demonstrated that exogenous H2O2 promotes an imbalance between production and removal of ROS towards the pro-oxidative state, often referred to as oxidative stress
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
