Resveratrol protects against bleomycin-induced lung injury in mice

Abstract

Objective To study the protective effects of the resveratrol on bleomycin-induced lung injury in mice and its underlying mechanism.Methods Male ICR mice were randomly divided into four groups(n=10):control group,bleomycin group,resveratrol group,and bleomycin+resveratrol group.Mice were anesthetized and infused intratracheally with 3mg/kg bleomycin to create lung injury model.Resveratrol(0.3mg/kg)was administered intraperitoneally before bleomycin infusion. The severity of pulmonary injuries was evaluated 24hafter inducing lung injury.H-E staining was used to assess the lung pathology.The levels of lipid peroxidation marker malondialdehyde(MDA),neutrophil infiltration marker myeloperoxidase(MPO)activity,and cytokines IL-6,IL-8,and IL-10were determined by commercial kits.Results Resveratrol treatment improved the bleomycin-induced lung injury in mice by significantly inhibiting the oxidative stress and decreasing lipid peroxidation marker MDA level(P0.01).Resveratrol treatment also attenuated the bleomycin-induced pulmonary infection by significantly decreasing MPO activity(P0.01)and the production of pro-inflammatory cytokines IL-6(P0.01)and IL-8(P 0.05)and increasing anti-inflammatory cytokine IL-10production(P0.01).Conclusion Resveratrol can protect against bleomycin-induced lung injury in mice,probably through its antioxidant and anti-inflammatory effects.