Resveratrol production from Polygonum cuspidatum by one-pot green extraction and bioprocessing of polydatin

Abstract

Polygonum cuspidatum is extensively utilized globally due to its abundant content of stilbene compounds in plant cells. In this study, we developed a one-pot green extraction procedure in situ that combined enzymatic hydrolysis of polydatin with extraction of resveratrol using a Deep Eutectic Solvent (DES) from Polygonum cuspidatum. Fifteen DESs were synthesized, consisting of choline-alcohols (n = 3), choline-organic acids (n = 5), choline-amines (n = 2), and choline-sugars (n = 5), and their productivity in combination with three types of enzymes were evaluated. Based on the total yield of resveratrol and polydatin and conversion rate of polydatin, β-glucosidase assisted choline chloride-glycol DES was selected. To maximize the yield of resveratrol, single-factor tests combined with response surface methodology were selected to optimize the extraction process. Under optimal extraction conditions, the extraction efficiency and yield of resveratrol were 99.74 % and 85.56 ± 0.96 µmol/g (19.53 ± 0.22 mg/g, n = 3), respectively. Additionally, β-glucosidase can still keep excellent enzyme activity in choline chloride-glycol DES at 64 °C with the 100 % of conversion rate of polydatin. This study offers a new, cost-effective, efficient, and environmentally friendly method for enhancing resveratrol production. Additionally, it showcases the applicability of enzyme-catalyzed extraction utilizing DES as a green and sustainable technique for available resveratrol from Polygonum cuspidatum.