Abstract
Objective To investigate the protective effects of resveratrol on kidney of uremic rats and to explore whether the mechanism is associated with heat shock protein 70 (HSP70) expression. Methods Sixty male Sprague Dawley rats were randomly separated into 5 groups, including sham group, uremic model group, and different doses of resveratrol group (5 mg/kg, 10 mg/kg, and 20 mg/kg). The serum creatinine (Cr) and urea nitrogen (BUN) levels were detected by Automatic Biochemical Analyzer (ABA). The pathological changes of renal tissues and the renal interstitial fibrosis were analyzed by hematoxylin-eosin (HE) and Masson, respectively. The expression of HSP70 protein in renal tissues was detected by immunohistochemistry. The expression of HSP70 and NF-κB pathway-related proteins were detected by Western blot. To further validate the protective role of resveratrol through activating HSP70 in uremic rats, HSP70 activator (17-AAG) and HSP70 inhibitor group (MKT-077) were used. Results In the model group, the levels of Cr and BUN in serum were significantly increased, and the renal interstitial collagen deposition was also obviously increased (p < 0.05). Compared with the model group, the levels of Cr and BUN in different doses of resveratrol groups were remarkably declined, and the renal interstitial collagen deposition was declined (p < 0.05). Resveratrol also significantly improved the renal tissue lesions when compared with the model group. In renal tissues, different doses of resveratrol treatment remarkably raised HSP70 and p-IκBα expression and also remarkably declined the level of p-P65 protein (p < 0.05). Meanwhile, the effect of 17-AAG was similar to 20 mg/kg resveratrol on NF-κB pathway-related proteins expression. After the added MKT-077 in the resveratrol treatment group, the levels of HSP70 and p-IκBα in the renal tissue were remarkably declined; however, the levels of p-P65 protein was remarkably raised (p < 0.05). Conclusion Resveratrol played a protective role on the kidney of uremic rats through activating HSP70 expression.
Highlights
Uremia is a serious complication of kidney disease syndrome, which developed alongside the deterioration of renal function [1]
The high levels of serum Cr and BUN in blood usually means the failure of renal tissue
These results suggested the protection role of resveratrol on kidney tissue in uremic rats
Summary
Uremia is a serious complication of kidney disease syndrome, which developed alongside the deterioration of renal function [1]. The most obvious feature is that uremia elevated concentrations of urea in the blood are involved with fluid, electrolyte, metabolic abnormalities, and hormone imbalances [2]. The number of uremic patients is ever-increasing with dysfunction of kidney; the treatments of uremic are still miserable. About 2 million people would die because of uremia [1]. Patients could keep alive by treating with kidney transplant and dialysis. These people suffer unbelievable pain with a higher rate of blood and heart problems [3]
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