Resveratrol chemoprotective effect and COX 2 down regulation in tumor suppression in DMBA induced breast cancer in female Sprague Dawley rats

Abstract

Background: Aim of the study was to assess the Chemo protective role of Resveratrol in 7,12‑Dimethylbenzanthracene (DMBA) induced breast cancer in Female Sprague Dawley rats and its possible role in down regulation of COX 2, an enzyme known to be expressed in breast cancer tissues.Methods: A total of 40 female Sprague dawley rats (total 4 groups, n = 10 per group) 6 weeks old, group 1 on pulverized rodent diet, group 2 DMBA with diet, group 3 DMBA and diet with Resveratrol 100mcg, group 4 DMBA and diet with Resveratrol 200mcg. After 120 days experiment was terminated and tumors were analyzed for multiplicity, incidence and histology. Cox 2 expression was analyzed by Western blot analysis. Values were statistically tested using one way variance and Tukey’s comparison test.Results: Body weight and tumor volume was similar, there was remarkable high latency period for tumor onset and reduction in tumor multiplicity andincidence in resveratrol treated groups. Tumor incidence was 42.27±10.17 for Group 2, 21.91±5.87 for Group 3, 13.73±3.98 for group 4. Tumor multiplicity was reported as 0.8909±0.30 for group 2, 0.1036±0.04 for group 3, 0.04545±0.02 for group 4. Histopathological analysis revealed ductal carcinoma in group 2, minor tissue necrosis in group 3 and fibroadenoma in group 4.Conclusions: Resveratrol has chemoprevention action against DMBA induced breast cancer and suppresses COX 2 expression in breast carcinoma.