Abstract
This study is aimed at developing a method that can inhibit resveratrol (Res) degradation in Dulbecco's modified Eagle medium (DMEM) and at evaluating the effects of Res on the replicative lifespan of Hs68 cells. We hypothesized that Res can extend the lifespan of Hs68 cells if we can inhibit the oxidative degradation of Res in the medium. We found that the addition of ≥5 U/mL SOD to the medium could completely inhibit Res degradation in DMEM. Fetal bovine serum (FBS) contained 29.3 ± 1.1 U/mL of SOD activity. FBS could prevent Res degradation in the medium through SOD activity and Res–FBS interaction, but the regular FBS concentration (i.e., 10% FBS) exhibited an insufficient ability to completely inhibit Res degradation. We found that pyruvate (1 mM) could potentiate SOD to scavenge superoxide at approximately 2.2-fold. Thus, 10% FBS combined with pyruvate (1 mM) could completely inhibit Res degradation. When Res was not degraded, it still shortened the lifespan of Hs68 cells. Overall, the proposed method involving 10% FBS combined with pyruvate (1 mM) could completely prevent Res degradation. However, in contrast to our hypothesis, Res could induce the shortening of the lifespan of Hs68 cells. The stability of Res analogs (i.e., oxy-Res and acetyl-Res) in the medium and their effects on the lifespan of Hs68 cells were also investigated.
Highlights
Resveratrol (Res) is a polyphenol that exists in grape skins and is present in red wine
We recently demonstrated that glucose restriction can mimic calorie restriction (CR) to extend the lifespan of human fibroblastic Hs68 cells [17]
Res and oxy-Res were undegraded in MEM with 10% Fetal bovine serum (FBS) and pyruvate (1 mM) under regular culture conditions (Table 5). These results suggested that the additional ingredients, including antibiotic and nonessential amino acids, exerted no effect on the ability of 10% FBS combined with pyruvate (1 mM) to inhibit Res degradation in Dulbecco’s modified Eagle medium (DMEM) and MEM
Summary
Resveratrol (Res) is a polyphenol that exists in grape skins and is present in red wine This micronutrient possesses antioxidant, anti-inflammatory, antidiabetic, anticancer, and cardiovascular protective properties [1, 2]. A calorie restriction mimetic (CRM) is a compound that mimics the metabolic, hormonal, and physiological effects of calorie restriction (CR), activates stress response pathways observed in CR, enhances stress protection, produces CR-like effects on longevity, reduces age-related disease, and maintains youthful function [3]. All of these actions occur without significantly reducing food intake, at least initially [3]. We aimed to clarify the CRM potential of Res by examining whether Res can extend the lifespan of Hs68 cells
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