Abstract
Resveratrol (Res) is a multi-functional polyphenol compound that has protective functions in cardiovascular and neurodegenerative diseases. This study aimed to determine the effect of Res on osteogenic differentiation and bone mineralization in zebrafish (Danio rerio) with dexamethasone (Dex)-induced bone damage. Our results showed that Dex exposure (15 μmol/l) decreased the green fluorescence areas and the integrated optic density (IOD) values in the skull bones of zebrafish larvae of the TG(SP7:EGFP) strain in a dose-dependent manner (p < 0.01). Furthermore, Dex exposure decreased the alizarin red S-stained areas (bone mineralization area) in the skeleton and spinal bones of zebrafish larvae of the AB strain in a dose-dependent manner (p < 0.01). By contrast, Res treatment (150 μmol/l) significantly increased both the green fluorescence and bone mineralization area in Dex-exposed zebrafish larvae. Thus, our data show that Res improves bone mineralization after glucocorticoid-induced bone damage in a zebrafish model. Res may be a candidate drug for the prevention of osteoporosis.
Highlights
Resveratrol (Res; 3,4′,5-trihydroxystilbene) is a multifunctional polyphenol found in the seeds of plants, such as grapes (Polonio et al, 2014), peanuts (Xiong et al, 2014), giant knotweed, and cinnamon (Di Benedetto et al, 2018)
Natural mating was allowed to occur between the following pairs of wild-type AB-strain zebrafish and TG(SP7:EGFP)-strain zebrafish (DeLaurier et al, 2010; Knopf et al, 2011): male and female TG zebrafish, male TG and female AB-strain zebrafish, female TG and Abbreviations: Dex, Dexamethasone; DMSO, Dimethyl sulfoxide; Dpf, Days post-fertilization; green fluorescent protein (GFP), Green fluorescent protein; glucocorticoid-induced osteoporosis (GIOP), Glucocorticoid-induced osteoporosis; integrated optic density (IOD), Integrated optical density; Res, Resveratrol; reactive oxygen species (ROS), Reactive oxygen species
The results showed that there was no significant difference in the size of each fish compared with each group (p > 0.05; Tables 1 and 2)
Summary
Resveratrol (Res; 3,4′,5-trihydroxystilbene) is a multifunctional polyphenol found in the seeds of plants, such as grapes (Polonio et al, 2014), peanuts (Xiong et al, 2014), giant knotweed, and cinnamon (Di Benedetto et al, 2018). Res is a plant antitoxin with antioxidant functions; it is produced in response to pathogens and adverse environmental changes. Res primarily exists in its glycoside form. It has two geometric isomers: cis and trans, which are usually present in plants with 3β-D2 glucosides. The removal of the glucosyl group from Res via hydrolysis leads to the formation of polydatin. Polydatin is degraded by glycosidases in the intestinal tract to release Res
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
