Resveratrol Alleviates Renal Inflammatory Injury In Exhaustive Exercise Rats Via Inhibiting Nlrp3 Inflammasome Activation

Abstract

PURPOSE:To investigate the effect of resveratrol on the amelioration of renal inflammatory injury in exhaustive exercise rats via inhibiting pyrin domain containing 3 (NLRP3) inflammasome activation. METHODS:36 male SD rats were randomly divided into 4 four groups, control group (Con, n = 8), resveratrol group (Rsv, n = 8), exhausted exercise group (Ex, n = 10), and exhausted + resveratrol group (Ex+Rsv, n = 10). Rats in Ex and Ex+Rsv groups performed exhausted treadmill exercise for 4 weeks. In Rsv and Ex+Rsv groups, resveratrol was orally administered daily at a dose of 50 mg/kgBW/d. Anesthesia treatment was taken 24 hours after the last training. Urine and renal were collected for detection. ELISA was used to detect urine neutrophil gelatinaseassociated lipocalin (NGAL) and kidney injury molecule-1(KIM-1) levels; the morphological and structural changes was observed, and the protein expression of NLRP3, ASC, caspase-1, interleukin (IL)-1β and IL-18 were detected by Western blot. RESULTS: Compared with Con group, Urine NGAL (11.01 ± 1.18 ng/ml vs. 7.48 ± 0.31 ng/ml) and KIM-1 (10.66 ± 1.39 pg/ml vs.2.83 ± 0.39 pg/ml)in Ex group were significantly higher(P < 0.01), HE light microscopy of renal in group Ex showed pathological changes in renal structure; the relative protein expression normalized to β-actin of NLRP3 (0.20 ± 0.06vs.0.56 ± 0.13), ASC (0.43 ± 0.07vs.0.18 ± 0.04) and caspase-1 (0.42 ± 0.07vs.0.13 ± 0.04) in Ex group were remarkbly increased(P < 0.01), and the same with the protein expression of IL-1β(0.42 ± 0.04vs.0.14 ± 0.05) and IL-18 (0.35 ± 0.02vs.0.18 ± 0.05) (P < 0.01); Compared with Ex group, resveratrol supplementation significantly down-regulated the levels of urine NGAL(9.10 ± 0.77 ng/ml vs. 11.01 ± 1.18 ng/ml) and KIM-1(6.07 ± 0.73 vs.10.66 ± 1.39 pg/ml), and also the relative protein expression normalized to β-actin of NLRP3(0.27 ± 0.10 vs.0.56 ± 0.13), ASC(0.24 ± 0.02 vs.0.43 ± 0.07) and caspase-1(0.20 ± 0.07 vs.0.42 ± 0.07) (P < 0.05), the morphological changes of renal caused by exhaustive exercise in rats was alleviated. CONCLUSIONS: Resveratrol can effectively alleviate the inflammatory renal damage of rats with exhaustive exercise, the mechanism may be related to the inhibition of resveratrol on the excessive activation of NLRP3 inflammasome.