Resumption of postpartum reproductive cyclicity and pregnancy rates after treatment with CIDR plus PGF2 α in normally calved and retained fetal membranes affected water buffaloes

Abstract

An experiment was conducted to compare the estrus induction response, estrus pattern, endometrial regeneration and pregnancy rates following treatment with CIDR plus PGF2 α between normally calved (NC) and retained fetal membranes (RFM) affected water buffaloes. A total of 32 water buffaloes including 16 treated for RFM and 16 NC were selected and divided into 4 groups as I and II, and III and IV respectively. Buffaloes of group I and III were dewormed with Albendazole followed by the administration of mineral mixture in feed for 15 days. Buffaloes of all the groups were treated with CIDR intravaginally for 9 days and they received PGF2 α at 24 hours prior to CIDR withdrawal. After CIDR removal, the buffaloes of all the groups were artificially inseminated during induced estrus. Those buffaloes returned to estrus following breeding at induced estrus, were artificially inseminated twice at an interval of 24 hours. The estrus response following CIDR withdrawal was 100%. The RFM affected buffaloes had significantly longer interval to onset of estrus and shorter duration of estrus than NC buffaloes. More pronounced intensity of estrus following CIDR treatment was observed in NC buffaloes than in RFM affected buffaloes. The mean pregnancy rates obtained in NC and RFM affected buffaloes were 81.25% and 50%. Complete loss of epithelial layer, degenerative changes of endometrium and atrophic changes in the endometrial glands were observed in RFM affected buffaloes before estrus induction. After treatment, RFM affected buffaloes showed clear regeneration of endometrium and its glandular acini. It is concluded that estrus induction using CIDR plus PGF2 α might help to augment the fertility in NC and RFM affected buffaloes at field level.