Results Regarding In Vitro Proliferation in Goji (Lycium barbarum)

Abstract

SUMMARY At the Fruit Research Station of Cluj experiments were done for the in vitro propagation of an important fruit shrub, Lycium barbarum. An experimental series was carried out in which MS basal medium was used, with different concentrations of growth regulators (BAP at 0.2 and 0.5 mg/l or Kinetin at 0.2 and 0.5 mg/l. All the variants were gelled with 6 g/l Plant Agar from Duchefa. On these variants the plant material generated callus and growth was inhibited, and the multiplication rate was zero. Another experimental series was carried out, in which Murashige & Skoog 1962 medium with 0.5 mg/l BAP was used, with the pH adjusted to 5.8 and 50 g/l starch was used as gelling agent. 2-node stem fragments were used as microcuttings, 7 pieces/vessel. The inoculi proliferated intensely and after a 2 month culture cycle 191 shoots resulted/vessel (an average of 27.29/plantlet), of which 174 were short, 0.5-2 cm in length and 17 were of 3-4 cm. Because some of the shoots could be fragmented in 2 microcuttings each, 221 microcuttings resulted/vessel, that is, a multiplication rate of 31.57 microcuttings/plantlet.